Berberine enhances the function of db/db mice islet β cell through GLP-1/GLP-1R/PKA signaling pathway in intestinal L cell and islet α cell

Background: The evidence on berberine stimulating the secretion of GLP-1 in intestinal L cell has been studied. However, few research explored its role generating islet α cell. Our experiment aims to clarify mechanism promoting and cell, activating GLP-1R downstream molecules through endocrine paracrine ways, thus improving function β treating T2DM. Methods: After confirming that can lower blood glucose improve insulin resistance db/db mice, identity maintenance, proliferation apoptosis cells were detected by immunohistochemistry immunofluorescence. Then, activation GLP-1/GLP-1R/PKA signaling pathway was evaluated Elisa, Western blot PCR. Finally, this verified vitro experiments Min6 cells, STC-1 aTC1/6 cells. Results: Berberine ameliorates metabolism mice. Additionally, it also increases number enhances This process is closely related activate GLP-1R/PKA autocrine paracrine, increase expression molecule such as GLP-1, GLP-1R, PC1/3, PC2, PKA, Pdx1. In , phenomenon enhanced signal had observed, which confirmed results animal experiments. Conclusion: maintain normal